The Effect of Inoculum Size and Medium Volume on Methionine Production by Bacillus species EZ-13 and ZM-10

The effect of inoculum size and medium volume on methionine production by Bacillus species EZ- 13 ZM-10 was studied. methionine-producing bacteria had already been isolated from within Anambra State University (now Chukwuemeka Odumegwu Ojukwu University), Uli campus State. They were purified identified as EZ-13 using cultural biochemical characteristics. Thereafter, they used to evaluated the influence in 250 ml flasks containing 50 sterile fermentation medium. findings revealed that ZM-10, at 4% v/v, recorded maximum yields 2.8 mg/ml 2.1 respectively. concentrations both decreased beyond v/v. stimulated yield 3.0 2.4 ZM-10. for ml. result study showed varying sizes volumes, could fermentation.